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Synthetic BiologyMetabolic EngineeringDirected EvolutionSaccharomyces CerevisiaeEscherichia ColiGenetic CircuitsProtein EngineeringGenetic CircuitGenome EngineeringGene ExpressionGENOME EDITINGGene CircuitsProtein SynthesisHeterologous ExpressionCell-free Synthetic BiologyDNA AssemblyEngineered Escherichia ColiTranscription and TranslationControl of Gene ExpressionCell-free Expression